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KMID : 0366220160510030181
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Korean Journal of Hematology
2016 Volume.51 No. 3 p.181 ~ p.186
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Detection of MYD88 L265P in patients with lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia and other B-cell non-Hodgkin lymphomas
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Shin Sang-Yong
Lee Seung-Tae
Kim Hyun-Young
Park Chang-Hun
Kim Hee-Jin
Kim Jong-Won
Kim Seok-Jin
Kim Won-Seog
Kim Sun-Hee
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Abstract
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Background: Recent studies have identified a high prevalence of the MYD88 L265P mutation in lymphoplasmacytic lymphoma (LPL)/Waldenstrom macroglobulinemia (WM) cases, whereas low frequencies have been observed in other B cell non-Hodgkin lymphomas (NHLs).
Methods: We evaluated the sensitivity of the mutant enrichment 3'-modified oligonucleotide (MEMO)-PCR technique, a new detection method. We examined the MYD88 L265P mutation in a series of Korean patients with LPL/WM and other B cell NHLs in bone marrow aspirates, using the MEMO-PCR technique.
Results: The sensitivity of MEMO-PCR was estimated to be approximately 10-16.7%. MYD88 L265P was detected in 21 of 28 LPL cases (75%) and only three of 69 B cell NHL cases (4.3%).
Conclusion: Although MEMO-PCR had relatively low sensitivity, we confirmed the high prevalence of the MYD88 L265P mutation in Korean LPL patients. Our study suggests the diagnostic value of MYD88 L265P for differentiating B-cell NHLs.
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KEYWORD
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Lymphoplasmacytic lymphoma, MYD88 L265P, Aspirate, MEMO-PCR
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